BUTYRIC ACID DELIVERY MATTERS: OLUS®C4 IN POULTRY AND SWINE

In poultry and swine production, gut-health conversations often start when the signs are already there. Wet litter. Uneven flocks. Poor starter intake. Post-weaning setbacks.

By that point, the pressure may have been building for days.

The practical question is not only which additive is added to the ration. More relevant is whether the functional component is delivered in a way that fits the animal, the phase and the challenge. That question becomes even more important in markets where heat, humidity, raw material variation, feed cost pressure and antibiotic-reduction programs all meet in the same ration.

ENERGY UTILIZATION IN LOW ENERGY DIETS

You want to unlock the energy that is already in your fat-rich diet. In poultry and swine production, efficient fat digestion remains a key determinant of feed cost control and biological performance. Fat is the most energy-dense and expensive component in many formulations. However, its nutritional value depends entirely on how effectively the animal can digest and absorb it.

Young animals in particular often show limitations in fat utilization due to an immature digestive system and relatively low endogenous bile secretion. Under these conditions, part of the dietary fat may pass through the intestine unabsorbed, contributing to energy losses, greasy manure, and variability in performance.

 

The challenge

Why fat digestibility is your most important control variable

In poultry nutrition, efficient fat digestion is the difference between a high-performing flock and wasted feed costs. Fat is the most expensive energy source in a poultry ration. However, young birds, especially broiler chicks, struggle to process dietary fats efficiently.

Problem

Young chicks have an immature digestive system with low bile secretion. Their natural bile salt production simply cannot keep up with modern high-energy formulations, leaving 20-30% of dietary fat undigested and wasted.

 

The science of fat absorption

How fats are utilized: a three-phase process

For dietary fat to be utilized, it must pass through three physiological steps:

  1. Emulsification
    Large fat globules are broken into smaller droplets, increasing the surface area available for enzymatic action.
  2. Hydrolysis
    Lipase enzymes split triglycerides into monoglycerides and free fatty acids, molecules the intestine can actually absorb.
  3. Micelle formation
    These digestion products are incorporated into micelles that transport lipids across the intestinal wall into the bloodstream.

When emulsification is suboptimal, the entire downstream process becomes less efficient, especially in young birds and piglets or in diets rich in saturated fats. Birds produce natural bile salts for emulsification, but young chicks’ immature systems create a bottleneck. Standard micelle formation cannot bridge this gap because it lacks the chemical structure for efficient micelle formation in the aqueous (water-based) environment of the chick’s gut.

 

Not all lysolecithins are the same

While many products are marketed under the broad umbrella of “lysolecithins,” their efficacy in a broiler’s gut depends heavily on their chemical “fingerprint.”

The quality and performance of a lysolecithin product are generally dictated by three main factors: the degree of conversion, the phospholipid profile, and the carrier system

  1. The Degree of Hydrolysis: Standard lecithin is a mixture of phospholipids (PC, PE, PI). To create lysolecithin, an enzyme (phospholipase A2) snips off one fatty acid chain.
    • Partial Hydrolysis: If the conversion is low, the product behaves more like standard lecithin—good for food texture, but weak for fat digestion in a bird.
    • High Hydrolysis: A high conversion rate creates a higher concentration of lysophospholipids, which are significantly more “water-loving” (hydrophilic) and effective at forming the small micelles needed for absorption.
  2. The HLB Value (Hydrophilic-Lipophilic Balance): This is the “gold standard” for measuring emulsifier strength.
    A table of parameters in standard lecithin and high-quality lysolecithin
  3. The Phospholipid Profile
    Not all “Lyso” molecules are equal. The specific ratio of the following determines the biological activity:
    • Lyso-Phosphatidylcholine (LPC): The “heavy hitter” for improving membrane permeability and nutrient absorption.
    • Lyso-Phosphatidylethanolamine (LPE): Excellent for helping stabilize the emulsion.
    • Lyso-Phosphatidylinositol (LPI): Often plays a role in cell signaling within the gut wall.

Overview of the four key phospholipids in lysolecithin. A explanation about LPC, LPE, LPA, LPI and their relative importance and primary role in animal nutrition.

Crucial Difference: Some lower-grade products are simply “modified lecithins” or blends with high amounts of carrier oil. High-tier lysolecithins maximize the LPC content, which is the most expensive but most effective component for poultry growth.

 

Why Olus®Digest is different

The lysolecithin advantage

While birds produce natural bile salts for emulsification, young chicks often have an immature digestive system with low bile secretion. Olus®Digest differs from standard soy lecithin because it has undergone enzymatic hydrolysis (removing one fatty acid chain).

Micelle formation after use of Elan Digest.

 

Key technical benefits

  • Higher HLB Value: Olus®Digest has a higher Hydrophilic-Lipophilic Balance (HLB) than standard lecithin. This makes it much more effective at stabilizing oil-in-water emulsions in the gut.
  • Smaller Micelles: It facilitates the formation of smaller, more stable micelles, which speeds up the absorption of fat-soluble vitamins (A, D, E, K) and pigments.
  • Membrane Permeability: Beyond emulsification, Olus®Digest can modify the fluidity of the intestinal cell membranes, making it easier for nutrients to pass through.

 

Impact on poultry performance

 

Practical application

Olus®Digest is most effective in:

  • Starter Diets: Where bile salt production is the limiting factor.
  • High-Density Diets: For broilers requiring rapid growth.
  • Saturated Fat Diets: When using vegetable acid oils or animal fats that are naturally harder to emulsify.

Efficient fat digestion is not guaranteed by fat inclusion alone. It depends on the animal’s physiological capacity, diet composition, and the effectiveness of the emulsification process in the gut. Well-characterized lysophospholipids can support this process. Olus®Digest is developed to help nutritionists improve fat utilization consistency under modern commercial conditions.

 

 

Muhammad Suleman, a Pakistani man with black hair wearing a black shirt with an Olus logo, in front of a sustainability-themed feature wall in the office at Hasselt the Netherlands.

About the author

Muhammad Suleman – Regional Manager | Southeast Asia and Middle East 

Muhammad Suleman, Regional Manager for Southeast Asia and the Middle East, joined the Olus team in 2023. His involvement with Olus brings a wealth of experience and a deep passion for the poultry industry. With years of experience and extensive knowledge of the complexities of poultry farming, Muhammad has a deep understanding of the market. Within Olus, he is actively involved in developing new feed additives and exploring nutritional strategies to improve gut health and performance in a sustainable way.

Reach out to Muhammad today for personalized guidance.

 

UNIVERSITY RESEARCH: ANTIBACTERIAL EFFECTS OF OLUS®C12

Glycerol monolaurin is a monoglyceride with strong antibacterial effects, mainly against Gram positive bacteria like Staphylococcus spp., Enterococcus faecalis, and Clostridium perfringens, while being less effective against Gram negative bacteria like E. coli and Salmonella spp.

Glycerol monolaurin is the active ingredient of Olus®C12, which is used to improve intestinal health by controlling the growth of potential pathogens in the small intestine of monogastric animals. In this in vitro experiment, the minimal inhibitory concentration of Olus®C12 was determined at NPChemBio Co., Ltd. in South Korea.

 

Materials & methods

  • Olus®C12 90% was dissolved in 99.9% ethanol prior to use.
  • Each of three bacteria (Streptococcus pneumoniae, Staphylococcus aureus and Clostridium perfringens) were inoculated onto a tryptic soy agar plate and incubated for 24h at 37°C. Single colonies were harvested and inoculated into a tryptic soy broth and incubated for 24h at 37°C. Bacterial growth was assessed by measuring the optical density (OD) at 600 nm using a spectrophotometer. A bacterial suspension was prepared by adjusting the culture to approx. 5×105 CFU/mL.
  • The test sample with Olus®C12 was serially two-fold diluted. 96 μL broth and 4 μL of the diluted sample were mixed and added to the wells of a 96-well microtiter plate. Streptomycin (10 μg/mL) was used as positive control, and 4 μL ethanol (without the test material) as negative control. Wells with only broth without bacteria or test material were used as blanks for OD correction. The microtiter plates were incubated for 24h at 37°C. After incubation, the contents of each well were thoroughly mixed to resuspend the bacteria, and the OD was measured at 600 nm. Finally, the OD values were corrected for the blank, and used to calculate bacterial growth inhibition. The minimum inhibitory concentration (MIC) is defined as the lowest concentration that achieves 100% growth inhibition.

In vitro results showing the effect of Elan®C12 on three bacterial species: Streptococcus pneumoniae, Staphylococcus aureus, and Clostridium perfringens.

* Product name updated from Elan® to Olus®.

In each bar graph, the positive control is given in green. Bacterial growth inhibition is shown, starting with the highest concentration of Olus®C12 at the right hand side of the bar graph, whereas the concentration is halved with each bar moving to the left. The lowest concentration that achieves full growth inhibition is the MIC value.

 

Results

Each test revealed a clear inhibition curve: the higher the concentration, the stronger the effect.

  • Clostridium perfringens: 99% inhibition at 35 µg/mL.
  • Streptococcus pneumoniae: 99% inhibition at 8.75 µg/mL.
  • Staphylococcus aureus: 99% inhibition at 20 µg/mL.

These MIC values are referring to the Olus®C12 product (90% GML concentration) that was used as the test substance. No MIC values could be determined for Salmonella gallinarum, Salmonella typhymurium and E.coli, up to the highest concentration tested of 70 μg/mL, 70 μg/mL and 1.48 mg/mL. Therefore, no results are reported on these Gram negative bacteria.

 

Practical relevance

Based on these MIC values, it can be calculated that a dietary dose level of Olus®C12 60% of 500 g/ton feed (i.e. 300 μg GML/mL) is adequate to limit growth of these Gram positive bacteria in the intestinal tract (assuming a water to feed ratio of 2:1).

 

Conclusion

A dietary dose level of Olus®C12 60% of 500 g/ton feed is adequate to limit growth of Streptococcus pneumoniae, Staphylococcus aureus and Clostridium perfringens in the intestinal tract, whereas no MIC against Gram negative bacteria could be determined.

Learn more about how Olus®C12 can support performance — contact our team today.

 

Professor Jan Dirk van der Klis, man met krullend haar en ronde bril die een zwarte Olus blouse draagt voor een natuurlijke groene wand.About the author

Prof. Jan Dirk van der Klis – Consultant Animal Nutrition

Jan Dirk van der Klis is a renowned poultry consultant with a PhD in Animal Nutrition from Wageningen University. Throughout his career, Jan Dirk has made noteworthy contributions to the industry. With his extensive expertise, he is offering valuable nutritional guidance to enhance the health and productivity of poultry. Jan Dirk’s profound knowledge and experience are pivotal in meeting the specific nutritional requirements of poultry and devising effective feeding strategies to yield optimal results. S

 

TRIBUTYRIN FOR LAYING HENS: SUPPORT EGG PRODUCTION

Ever wondered how poultry farmers help laying hens perform well and produce high-quality eggs consistently? One of the exciting tools in modern poultry nutrition is Olus®C4, a tributyrin-based feed additive. Tributyrin might sound complex, but its role in poultry diets makes it clear why it’s used to support laying hens.

Muhammad spends much of his time on poultry farms. He talks to farmers, works with nutritionists, and sees what works in the field. With this hands-on perspective, he follows closely how feeding strategies influence laying hens over time. Today, he explains what tributyrin is, how it’s used in poultry feed, and why he believes Olus®C4 is worth considering in a laying hen nutrition programme. 

SUPPORT PERFORMANCE IN AGP-FREE BROILER FARMING

In the high-pressure environment of intensive poultry farming, gut health directly affects performance. But how can gut health be supported without antibiotics? With growing awareness about sustainable farming, more producers choose for AGP-free farming and are turning away from antibiotic growth promoters (AGPs) and chemical additives.

To support this shift, Olus conducted a field trial in collaboration with the University of Agriculture in Faisalabad (Pakistan). They tested the effects of Olus®CX Liquid: a natural (phytogenic) feed additive added directly to broiler drinking water. In this blog you’ll discover how it improves feed efficiency, gut health, and bird immunity — making it a valuable tool in AGP-free poultry production.

 

Trial setup

Materials and methods

Seven treatment groups were evaluated, receiving Olus®CX Liquid through their drinking water at dosages of 350, 500, and 750 ml per 1,000 litres of water, applied between day 19 and 30 of age.

Note: All birds were fed an AGP- and coccidiostat-free diet (no antibiotics or anticoccidial drugs).

About the research team

This trial was conducted under practical field conditions by:

  • Dr. Waseem Abbas (Ph.D.) – Principal Investigator
  • Muhammed Hannan – Student at M. Phil. Animal nutrition

They tested Olus®CX Liquid under field conditions, making the results highly relevant for commercial broiler operations.

 

Results

Which improvements were seen?

The best performance was observed in groups T4 and T5, which received 500 ml per 1,000 litres of drinking water. The group with a lower dose (T3: 350 ml over a longer period) also came close.

Key improvements included:

  • Better feed intake and growth
    Broilers consumed more feed and gained more body weight.
  • Healthier gut microbiota
    The levels of good bacteria (Lactobacillus and Bifidobacterium) increased, while harmful bacteria like E. coli, Salmonella and Clostridium decreased.
  • Reduced signs of coccidiosis
    Levels of the common parasite Eimeria tenella were found in lower amounts, reducing the risk of gut damage.
  • Stronger gut structure
    Microscopic analysis showed better villus length and villus-to-crypt ratio, meaning the gut was in healthier condition to absorb nutrients.
  • Stronger immune response
    Birds showed higher levels of antibodies against common poultry viruses like IBV and NDV.

 

What is Olus®CX liquid?

Olus®CX Liquid is a phytogenic-based feed additive. That means it contains plant-derived ingredients known to support digestion and immunity. The different natural molecules work synergistically with each other to boost feed/water intake, improve immunity and help birds overcome critical challenges.

Recommended dossages

If you’re raising AGP-free broilers , Olus®CX Liquid can be a helpful part of your program. Based on different recent trials, this product works best at:

  • 500 ml per 1,000 L drinking water from day 19–23, or
  • 350 ml per 1,000 L from day 19–30 (if used over a longer period)

These protocols are easy to apply on-farm and fit well in AGP-free systems.

 

 

About the author

Muhammad Suleman – Regional Manager | South East Asia and Middle East 

Muhammad Suleman, Regional Manager for Southeast Asia and the Middle East, joined the Olus team in 2023. His involvement with Olus brings a wealth of experience and a deep passion for the poultry industry. With years of experience and extensive knowledge of the complexities of poultry farming, Muhammad has a deep understanding of the market. Within Olus, he is actively involved in developing new feed additives and exploring nutritional strategies to improve gut health and performance in a sustainable way.

Reach out to Muhammad today for personalised guidance on integrating Olus®CX Liquid into your strategy.

STIMULATING BROILER PERFORMANCE: NEW TRIAL RESULTS ON OLUS®CONTROL C12

Colonization of the intestinal tract by (potential) pathogenic bacterial in young broilers can lead to increased mortality and reduced production performance, reduced efficiency of nutrient digestibility and utilization, bone disorders, wet litter problems and welfare problems. Different feed additives like organic acids and their monoglycerides, botanicals, pre- and probiotics can be used to prevent pathogen colonization of the intestinal tract. Olus®Control is formulated as such a product with a strong antibacterial efficacy against Gram+ and Gram- bacteria, based on monoglycerides of four different fatty acids (C3, C4, C8 and C10) and essential oils. Olus®Control C12 is further enriched with monolaurin to enhance the immunocompetence of broilers.

The effect of this unique blend of active ingredients was tested in a broiler experiment under ‘commercial’ conditions. In February, we shared results showing significant improvements in body weight gain and feed conversion ratio (FCR) over a 35-day period. Now, we present a follow-up study with some new insights.

THE IMPACT OF HEAT STRESS ON GUT HEALTH IN CHICKENS

Heat stress is a significant challenge for poultry producers worldwide, especially in warmer climates. While the effects on egg production and growth are well-documented, the impact on chicken gut health is often overlooked. A healthy gut is crucial for nutrient absorption, immune function, and overall well-being. When chickens experience heat stress, their gut health can be severely compromised, leading to a cascade of negative consequences.

STIMULATE BROILER PERFORMANCE WITH OLUS®CONTROL C12

Colonization of the intestinal tract by (potential) pathogenic bacterial in young broilers can lead to increased mortality and reduced production performance, reduced efficiency of nutrient digestibility and utilization, bone disorders, wet litter problems and welfare problems. Different feed additives like organic acids and their monoglycerides, botanicals, pre- and probiotics can be used to prevent pathogen colonization of the intestinal tract. Olus®Control is formulated as such a product with a strong antibacterial efficacy against Gram+ and Gram- bacteria, based on monoglycerides of four different fatty acids (C3, C4, C8 and C10) and essential oils.
Olus®Control C12 is further enriched with monolaurin to enhance the immunocompetence of broilers. The effect of this unique blend of active ingredients was tested in a broiler experiment under ‘commercial’ conditions.

PHYTOGENIC FEED ADDITIVES AS SUPPLEMENTATION TO SUPPORT COCCIDIOSIS VACCINATION IN POULTRY

Coccidiosis is a common and challenging disease in poultry, significantly impacting bird health and performance. At Olus, we research and develop innovative solutions to mitigate these effects. By carefully selecting natural ingredients and leveraging extensive research and practical experience, we create products that not only support animal welfare but also help boost the growth of your business. This article delves into how phytogenics can help control coccidiosis by boosting immunity, reducing secondary bacterial infections, and improving nutrient digestibility. We also highlight our phytogenic feed additives, Olus®Biotic and Olus®CX, which leverage the power of essential oils to support gut health and overall poultry performance. 

CONTROL SALMONELLA AND E.COLI IN BROILERS

Poultry health and productivity face significant challenges due to the prevalence of pathogenic bacteria, such as Escherichia coli (E. coli) and Salmonella. These pathogens, commonly found in the gut of poultry, are classified as opportunistic, meaning they exploit weakened immune systems to cause disease. Olus has developed Olus®AC34, an advanced liquid feed additive designed to support a healthy and balanced gut, with strong antibacterial activity against Gram-negative bacteria like E. coli and Salmonella.